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Prepare dilution series and spread plate count Escherichia coli culture

FOOD802 Tasks for Practical Classes
Day 1:
1 Prepare dilution series and spread plate count Escherichia coli culture
2 Spread plate serial dilutions.
3 Inoculate dilutions of E. coli culture into Soleris, set incubator at 37 ºC
4 Inoculate dilutions of E. coli culture into BacTrac, set incubator at 37 ºC
Day 2:
1 Protocol 2 plate scanner. Compare results from manual and counting done by the Protocol 2 plate scanner.
2 Plot calibration curves for microFoss and BacTrac
ABSTRACT, SENTENCE ON AIM , METHODS ,RESULTS, CONCLUSIO
INTRODUCTION: ABOUT EACH DIFF METHODS (WHERE THEY USED, BACTRAC AND Soleris ), AIM OF THE LAB REPORT IN BULLET.(RAPID METHOD TO ENUMERATE BACTERIA)
MATERIAL AND METHODS: SERIAL DILUTION, .1% PEPTONE
PLATING : MacKonkey agar plate
BACTRAC: TRYPTIC SOY BROTH (TSB)
SOLERIS: COLIFORM SOLERIS MEDIA
INCUBATED: 37 °C
RESULT: TEST BEFORE TABLE…..EXPLANATION ABOUT THE GRPAH. THEN FIGURE
DISCUSSION
CONCLUSION: DISCUSS THE RESULT..THE FOUND IS GOOD OR BAD .. BACTRAC DIDN’T WORK..NOT FRESH MEDIA
WILL U RECOMMEND :BACTRAC HELP US TO GET THE RESULT FAST.
LIMIT: MEIDA AND ORGANISM (STAPHYLLOCOCCUS WE NEED TO STANDARDISE). ABOUT THE FOOD…NEED TO FOR CALIBARTION
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