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Pre-Lab Questions 1. A concentration gradient affects the direction that solutes diffusion. Describe how molecules move with respect to the concentration.

Pre-Lab Questions
1. A concentration gradient affects the direction that solutes diffusion. Describe how molecules move with respect to the concentration.
2. How does size affect the rate of diffusion? Consider the size and shape of a molecule in your response.
3. Does polarity affect the rate of diffusion? Explain your answer using scientific principles.
Experiment 1: Diffusion through a Liquid
Post-Lab Questions
1. Record your hypothesis from Step 3 here. Be sure to validate your predictions with scientific reasoning.
2. Which dye diffused the fastest?
3. Does the rate of diffusion correspond with the molecular weight of the dye?
4. Does the rate of diffusion change over time? Why or why not?
5. Examine the graph of diffusion. Does it match the data you recorded in Table 2? Explain why, or why not. Submit your own plot if necessary.
Experiment 2: Diffusion – Concentration Gradients and Membrane Permeability
Post-Lab Questions
1. Why is it necessary to have positive and negative controls in this experiment?
3. Which substance(s) crossed the dialysis membrane? Support your response with data-based evidence.
4. Which molecules remained inside of the dialysis bag?
5. Did all of the molecules diffuse out of the bag into the beaker? Why or why not?

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